Thwip!

Target creature gets +2/+2 and gains flying until end of turn. If it's a Spider, you gain 2 life.

Spider-Man 2099, Miguel O'Hara

When Spider-Man 2099 enters, return up to one target creature to its owner's hand.
Whenever one or more creatures you control deal combat damage to a player, draw a card.

Sensational Spider-Man

Whenever Sensational Spider-Man attacks, tap target creature defending player controls and put a stun counter on it. Then you may remove up to three stun counters from among all permanents. Draw cards equal to the number of stun counters removed this way.

Spectacular Tactics

Choose one —
• Put a +1/+1 counter on target creature you control. It gains hexproof until end of turn.
• Destroy target creature with power 4 or greater.

Path to Exile

Exile target creature. Its controller may search their library for a basic land card, put that card onto the battlefield tapped, then shuffle.

Web Up

When this enchantment enters, exile target nonland permanent an opponent controls until this enchantment leaves the battlefield.

SP//dr, Piloted by Peni

Vigilance
When SP//dr enters, put a +1/+1 counter on target creature.
Whenever a modified creature you control deals combat damage to a player, draw a card. (Equipment, Auras you control, and counters are modifications.)

Spectacular Spider-Man

Flash
{1}: Spectacular Spider-Man gains flying until end of turn.
{1}, Sacrifice Spectacular Spider-Man: Creatures you control gain hexproof and indestructible until end of turn.

Daily Bugle Building

{T}: Add {C}.
{1}, {T}: Add one mana of any color.
Smear Campaign — {1}, {T}: Target legendary creature gains menace until end of turn. Activate only as a sorcery.

Amazing Alliance

Creatures you control get +1/+1.
Whenever you attack with one or more legendary creatures, you gain that much life.

Lyla, Holographic Assistant

Whenever you draw a card, put a +1/+1 counter on target creature.

Spider-Suit

Equipped creature gets +2/+2 and is a Spider Hero in addition to its other types.
Equip {3} ({3}: Attach to target creature you control. Equip only as a sorcery.)

City Pigeon

Flying
When this creature leaves the battlefield, create a Food token. (It's an artifact with "{2}, {T}, Sacrifice this token: You gain 3 life.")

Origin of Spider-Man

(As this Saga enters and after your draw step, add a lore counter. Sacrifice after III.)
I — Create a 2/1 green Spider creature token with reach.
II — Put a +1/+1 counter on target creature you control. It becomes a legendary Spider Hero in addition to its other types.
III — Target creature you control gains double strike until end of turn.

Spider-Man, Peter Parker

Flying (This creature can't be blocked except by creatures with flying or reach.)
Whenever you gain life, put a +1/+1 counter on target creature you control. It gains indestructible until end of turn. (Damage and effects that say "destroy" don't destroy it.)

Aunt May

Whenever another creature you control enters, you gain 1 life. If it's a Spider, put a +1/+1 counter on it.

Selfless Police Captain

This creature enters with a +1/+1 counter on it.
When this creature leaves the battlefield, put its +1/+1 counters on target creature you control.

Spider-Bot

Reach
When this creature enters, you may search your library for a basic land card, reveal it, then shuffle and put that card on top.